Bacterial translocation has been suggested as a potential mechanism that leads to sepsis or multiple organ failure. In such a situation, there was no definite septic focus as a leading cause. To preove the evidence of bacterial translocation in
the
circumstance of mechanical in testinal obstruction, we studied the animal model, rats, which intestine were prepared mechanicaly obstruction at 5cm proximal to of the ileocecal valve artificially. Eighteen Wistar albino rats were separated into
three
groups control (A group) explored and only sampled 24 hours duration after mechanical intestinal obstruction (B group), 48 hours after mechanical intestinal obstruction (C group). Inferior vena caval blood was sampled as well as tissue from a
mesenteric
lymph node, liver, spleen and lung. All samples were cultured for bacteriologic study except for anaerobic species. We obtained the following results. In group A, there were no enteric bacteria cultured in any of the 6 sample rats. In group B,
only
one
rat is grew mesenteric lymph node. Escherichia coli. In group C, all six rats were culture of enteric bacteria to include, Escherichia coli, Proteus vulgaris and Proteus mirabilis in 2 samples of two rats. Translecated enteric bacterias were
found
at
mesenteric lymph node of 4 rats, liver of 3 rats, spleen of 2 rats, blood and lung of one rat. Comparing the group B and C, the more longer duration persisted after mechanical intestinal obstruction, the more aggravated bacterial translocation
occurred.
We concluded that bacterial translocation was induced by mechanical intestinal obstruction I animal model and discovered that Escherichia coli was the most commonly translocated organism and the mesenteric lymph node was the most common site of
translocation. However we could not correlate the clinical significance of bacterial translocation in the mechanical intestinal obstruction of human by this study. We must do more effort to discover the clinical importance of bacterial
translocation in
the mechanical intestinal obstruction and to improve the clinical outcome.
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